LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. HTRF Signal + 2 hours @ RT [Cortisol] ng/ml 10 µL Standard or Sample 5 µL Cortisol acceptor reagent 5 µL Cortisol donor antibody ADD READ & ANALYSESafire 2™ readers must be appropriately be configured for HTRF readout by setting up the measurement conditions in the “multilabeling” function of Magellan software. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. For more. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Incubate overnight at RT. For more information about HTRF. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. An example of a LanthaScreen kinase assay run on a BMG LABTECH plate reader can be seen in the application note " LanthaScreen TR-FRET tyrosine kinase and protein kinase C assay ". Read & analyse. VICTOR Nivo Multimode Plate Reader. Lysates are then transferred to the assay plate for the detection of total-BTK by HTRF. At its core, Synergy H1 is a monochromator-based multi-mode microplate reader with a quadruple grating architecture for robust performance and flexibility. Partnerships for delivering turn-key solutions. Incubate overnight at RT. All requiredThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. PLATE READER INTRODUCTION Selecting the right plate reader for your lab can be challenging. As shown in Fig. EGFR protein (PV3872, 0. (2) Forskolin activates the adenylyl cyclase enzyme and increases the intracellular level of cAMP. FOR HTRF CERTIFIED READER. These plates can be read on all HTRF certified readers, thus making their implementation in. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. Lysis buffer 1X for two-plate assay protocol on adherent cells Determine the amount of lysis buffer needed for the experiment. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Assay plates were incubated at RT and read at different time points thereafter on an HTRF ® certified. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed TNFα Antibodies ADD READ ANALYSE Incubate 2 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. 96-well culture plateHowever, time-resolved fluorescence (TRF & TR-FRET) assays are used in conjunction with fluorophores that have a longer half-life. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The CulturPlate is PerkinElmer’s microplate designed for cellular assays, tissue-culture treated and sterilized by gamma-irradiation to facilitate adherent cell growth. For more. AlphaLISA SureFire Ultra results are expressed as “Alpha counts”, and HTRF results were expressed as DeltaF, calculated according to CisBio instructions. FOR HTRF CERTIFIED. FOR HTRF CERTIFIED READER. Cover the plate with a plate sealer. 68. Plate 25 μL of cells in. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. Incubate overnight at RT. FOR HTRF CERTIFIED. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. The new ligands are divided into four groups according to their chemical structures and/or interactions with YTHDC1 (Table 1 and Fig. Choosing the right microplate reader ensures you’ll get an optimal readout. It enables you to gain insights you couldn’t achieve with detection measurements alone. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. The system provides even higher sensitivity for time-resolved fluorescence (TRF) applications. PerkinElmer’s 96w low volume, 96w and 384w microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. • Clone your plate data for multiple data reduction optionsExpanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. Nephelometers are dedicated instruments that can only detect scattering and are only available as standalone plate readers. • HTRF®-Certified Reader **. Table 1. The FLUOstar Omega is the ideal plate reader for life science applications. FOR HTRF CERTIFIED READER. Read & analyse. These plates can be read on all HTRF certified readers, thus making their implementation in. HTRF-specific. FOR HTRF CERTIFIED READER. Step 1 Step 2 Step 3 Step 4 Step 5. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. FOR HTRF CERTIFIED READER . The donor and acceptor can be used to label a variety of biomolecules, for. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. The LVis Plate contains 16 spots which can be loaded with 2 microliters of sample for subsequent absorbance measurements. PHERAstar FS Operating Manual BMG LABTECH Figure 6: Unlocking the plate carrier 3. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. FOR ORE INFORMATION Europe and other ountries 33(0)466 96 0 U. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Make sure to use the set-up for Eu Cryptate. Tecan Spark multimode microplate reader, with fluorescence Fusion Optics™ meets these challenges and offers everything you need to develop and run advanced biochemical and cell-based fluorescence assays. For more information. FlexStation® 3 Multi-Mode Microplate Reader. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. e. The optical systems use two scanningHighly compatible plates for HTRF. For more information. Optimization of the reader for the plate dimensions clearly improved the results as seen by the uniform signal (b), a lowered %CV of 2. Remove the plate sealer and read on an HTRF® compatible reader . Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. FOR HTRF CERTIFIED READER. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. 1) . Plate Type and Read Area should be selected based on the microplate used and wells to be read. As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Synergy NEO2 readers must be appropriately configured for HTRF® readout by setting up the measurement conditions in the Gen5™ Reader Control and Data Analysis Software. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. For more information about HTRF ®Highly compatible plates for HTRF. The HTRF cellular assays were found to be at least 16-fold more sensitive than the Western Blot. VICTOR® Nivo™ multimode plate reader 2 | For research use only. 2 Locking the Plate Carrier Upon closing the software or pushing the plate carrier in/out button for more than three seconds (figure 10), the plate carrier will move into the respective lock position. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. FOR HTRF CERTIFIED READER . After defining the objectives and their locations in Gen5: 1. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. HTRF Signal + 5 hours @ RT or . S. The optimized well design and high quality plastics provides low background and crosstalk. SERIAL DILUTIONS . Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. CLARIOstar is equipped with a specific optical device which enables the measurement of. Homogeneous Time-Resolved Fluorescence (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based) • Kinases and ATPases • Cytokine Assays TUNE Multi-Mode • cAMP Quantitation • Cell Migration • Cell Viability and Proliferation • Cytotoxicity • DNA/RNA Quantitation (Fluorescent) Homogeneous Time-Resolved Fluorescence (HTRF) . Microplate reader assays requiring infrared (IR) or. **For more information about HTRF® compatible readers and for set-up recommendations, please visit our website at:ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Configure your microplate reader. Advanced Phospho-ERK (Thr202/Tyr204) 2-plate assay protocol. These plates can be read on all HTRF certified readers, thus making their implementation in. Smallest and most affordable multimode plate reader in the industry. When MikroWin opens, if you already have a pre-existing template for. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. CLARIOstar is equipped with a specific optical device which enables the measurement of. Read & analyse. EX: HTRF Emission range. With this certification, Cisbio Bioassays guarantees that the Synergy H4 meets or exceeds strict performance and functional criteria for optimal HTRF® readout. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. pre+ 5 µL HTRFcompounds Activation Buffer. at room temperature STEP 4 Remove the plate sealer and read on an HTRF ® compatible reader. HTRF combines time-resolved fresh detection with FRET and is mainly used for drug screening in high-throughput. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. DLReady™ certified luminometer plate readers. PURCHASE SEPARATELY . The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. For more information about HTRF. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. HTRF KinEASE-TK kit and HTRF 96-well low volume plate were obtained from Cisbio Bioassays. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. FOR HTRF CERTIFIED READER. We recommend using a white plate for time-resolved fluorescence assays and other assays where a lower signal is to be expected, to reduce the signal from background autofluorescence. HTRF certified . The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. Remove the plate seal and read on an HTRF compatible reader. HTRF is compatible with a large range of available microplate readers. Incubate 4h at room temperature. Homogeneous Time-Resolved Fluorescence (HTRF) . HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. For more ® information about HTRF compatible readers and for set-up recommendations. Dual monochromators for assay flexibility With. Plate 25 μL of cells in. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:320/40 HTRF Eu cryptate 665/7uv Wide Filter 0. Plate 25 μL of cells in. For more information about HTRF ®The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The HTRF ratio obtained represents the total binding. At Cisbio we provide microplates that help you discover better. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. Designed to the highest standards to support your drug discovery research, Corning ® and Falcon ® microplates are ideal for drug discovery applications, including assay prep, assay development, and HTS and automation. It guarantees high level of cAMP accumulation in the cells. The laser beam passes through the sample well. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. Unlike fluorescence intensity which focuses on the quantification of emission intensity at a specific wavelength and neglects its polarization, fluorescence polarization specifically analyses as output the emission intensity of. Plate 25 μL of cells in. FOR HTRF CERTIFIED READER For more information about HTRF. For more information about HTRF. Typical applications The HTRF cartridge provides optimal performance for key applications. Read & analyse. White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes• HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. These are the different kinetic modes on BMG LABTECH microplate readers. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance forThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The LVis Plate is transparent in the UV-range of light and is compatible with all BMG LABTECH microplate readers. (donor) of compounds are measured on an HTRF-compatible reader. Each well generally requires 50µL of lysis buffer 1X. The kit includes reagents, buffers and a microplate. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. The reader only allows high performance for the used HTRF measurement when the HTRF is run with white plates. Not for use in diagnostic procedures. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detectionThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. For more. Plate 25 μL of cells in. GENERAL LAB WORK USING HTRF KIT 1 Plate 3 mL of cells in 6-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readers HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. For instance, when assay volumes are. 3. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. FOR HTRF CERTIFIED READER. It continues to deliver optimal results. The measurement of HTRF assays is predominantly performed on microplate readers. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesCertified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. Make sure the setup for Tb Cryptate is used. FOR HTRF CERTIFIED READER For. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. PerkinElmer. Incubate 4h at RT. This protocol, HTS designed, allows. Make sure the setup for Eu Cryptate is used. 1) . Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. Discover our high performance reader. Both readers demonstrated their ability to perform the cAMP G s HiRange assay with results well within Cisbio’s required. Make sure the setup for Tb Cryptate is used. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. 6% for 1,408 wells and a Z’ value of 0. Highly compatible plates for HTRF. For more information. Make sure the setup for Eu3+ Cryptate is used. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Discover the features and benefits of the VICTOR Nivo multimode plate reader, the smallest and most versatile instrument for life science applications. After an overnight incubation, the plate is washed 3 times. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Plate cells Stimulate cells 3. Read & analyse. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. For multiplexed luminescent and fluorescent assays, using a white plate will support maximum light output signal for the luminescent portion of the assay, but result in higher crosstalk and background for the fluorescent portion of the assay. Conclusion. FOR HTRF CERTIFIED. View Brochure. </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). Phospho-Rb (Ser780) two-plate assay protocol. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. • Low volume (20µl) • Compatible with all HTRF readers • For cell-based or biochemical assays Trying is believing. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4 SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. When equipped with a quad monochromator, the EnSight plate reader can improve performance for absorbance and fluorescence detection. For the standard curve described, all wells of the microplate are read. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. + 4 µL HTRF ® pre-mixed antibodies . Incubate overnight at RT. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. These plates can be read on all HTRF certified readers, thus making their implementation in. Your lab might even have one already. Plate Reader Requirement. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. This protocol gives the cells viability and confluence to be monitored. This protocol gives the cells viability and confluence to be monitored. (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based). Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. Make sure the setup for Eu Cryptate is used. It can also be further streamlined toLysates are then transferred ®to the assay plate for the detection of Total TYK2 by HTRF reagents. Bench top solutions for cell based assays. If not, our scientific team will help you choose the instrument that best suits your needs and budget. . Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Envision Nexus. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. In addition, stacker. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. For more ® information about HTRF compatible readers and for set-up recommendations, please. Figure 3. For more. Incubate overnight at RT. FOR HTRF CERTIFIED READER. Remove the plate seal and read on an HTRF compatible reader. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. FOR HTRF CERTIFIED READERThis makes the PHERAstar ®FSX the fastest laser-based TRF reader on the market: 8 seconds for a 96-well plate. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance forCisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Make sure the setup for Eu 3+ Cryptate is used. * For HTRF microplate recommendations, please visit binding of the fluorescent ligand to the receptor, TR-FRET occurs and is measured with an HTRF-certified microplate reader. 96-well culture plateThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same . Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. How HTRF can simplify your life The PHERAstar FSX is the best microplate reader for all HTRF assays: Sensitive, fast and reliable measurements with best Z´ and DeltaF% HTRF-certified with white and black plates; Dedicated excitation laser and detectors (PMTs) for best sensitivity; Simultaneous detection of donor and acceptor gives speed and precision See full list on bmglabtech. For more information. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersHTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Reagents were dispensed following Cisbio’s ligand binding protocol in 384-well format (white plates). But sometimes these parameters are not sufficient enough. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. HTRF-compatible SpectraMax Multi-Mode Readers. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. The PHERAstar ® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. NON-SPECIFIC BINDING TOTAL BINDING+ 4 µL HTRF pre-mixed antibodies. FOR HTRF CERTIFIED READER. 3nm, important when reading at 280 nm for DNA analysis, for more exact results. These convert photons generated by fluorescence or. No washing steps are required. FOR HTRF CERTIFIED READER. 10 µL Standard or Sample. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. Due to the chemical and photophysical. Part Number# Product Description Number of Plates# 66PL96001 HTRF 96 well low volume plate 1 plate 66PL96005 HTRF 96 well low volume plate 5 platesThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The adapterResults summary for HTRF IP1 standard curve. Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. It is typically used to determine the concentration of nucleic acids and proteins. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. VICTOR® Nivo™ multimode plate reader. It can also be further streamlined to TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate. Unlike standard ELISA plate readers, MSD. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The Dual-Luciferase ® Reporter Assay or DLR is widely used to study gene transcription and regulation. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. • Select Excitation Filter 320x40 HTRF Eu cryptate* • Select Excitation Optic Empty • Select Emission Filter 495xm10uv • Select Emission Optic 2 – FPThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. + 4 µL HTRF pre-mixed antibodies. Compared to ELISA technology, MSD assays: Require far less sample. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Lysates are then transferred to the assay plate for the detection of total cRaf by HTRF. Plate 25 μL of cells in. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm:. Data Analysis HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. One plate carrier adapter is provided with the instrument. Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate Antibody• HTRF®-Certified Reader. It measures at any wavelength, with top and bottom reading for most standard technologies. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Plate 25 μL of cells in. PROTOCOL AT A GLANCE. It can also be further streamlined to a one-. EnVision Nexus ultimode Plate Reader 2 Benefits • Provides the speed and sensitivity to boost your lab’s productivity with ultra-high throughput • Freely configurable and upgradable to adapt to your needs today and tomorrow • Fully automatable for walkaway convenience • Certified for use with HTRF® technology Key features BMG LABTECH plate readers certified for LanthaScreen detection include the PHERAstar FSX, CLARIOstar Plus and VANTAstar. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Make sure the setup for Eu Cryptate is used. TR-FRET was measured on the SpectraMax i3x and iD5 readers (see Table 1 for instrument settings). For more information about HTRF ® compatible readers and for set-up recommendations, please. best fluorescence polarization plate reader on the market. This DLReady-certified microplate reader can also come equipped with Simultaneous Dual Emission for BRET applications and can be further upgraded to a multi-mode reader. 4. To perform the assay, use white plate only. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. ® Detection of Progesterone Receptor with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Revvity. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. Lysates are then transferred to the assay plate for the detection of Phospho-VASP (Ser239) by HTRF. On BMG LABTECH´s NEPHELOstar Plus, a laser diode (at 635 nm) with a highly collimated beam is used as high-intensity light source. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. Revvity. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. ratio, high sensitivity, wide dynamic range, superior stability, and excellent flexibility. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same . HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. Incubate overnight at RT. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. Make sure the setup for Tb3+ Cryptate is used. The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. HTRF is a proprietary time-resolved fluorescence technology that overcomesThe company offers different HTRF-certified, multidetection microplate readers such as the FLUOstar Omega, POLARstar Omega, CLARIOstar and PHERAstar. EnVision Nexus ultimode Plate Reader 2 Benefits • Provides the speed and sensitivity to boost your lab’s productivity with ultra-high throughput • Freely configurable and upgradable to adapt to your needs today and tomorrow • Fully automatable for walkaway convenience • Certified for use with HTRF® technology Key featuresBMG LABTECH plate readers certified for LanthaScreen detection include the PHERAstar FSX, CLARIOstar Plus and VANTAstar. For more information about microplate recommendations, please visit our website • Store the kit at -16°C. No washing steps are required. CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . Incubate overnight at RT. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Push the screw down and turn it clockwise to lock the plate.